| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180559 |
| Chromosome: | chromosome 16 |
| Location: | 1927765 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656150 | (1 of 1) PTHR23504:SF1 - PROTEIN T25D3.4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCAGGGGCAAGACCAGAAGCTTACAG |
| Internal bar code: | GCGCTTTAATCGATGGACCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 99.1184 |
| LEAP-Seq n confirming: | 4722 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCGACCTGCTCATCCTC |
| Suggested primer 2: | TAGACGAGTAGGCGCGATTT |