| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180921 |
| Chromosome: | chromosome 16 |
| Location: | 2276850 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659050 | (1 of 1) 4.2.99.20 - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / SHCHC synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGACGGCGGGCTCTGGTCACAGGCGGC |
| Internal bar code: | TTTTGATTTTGGTTCATACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 99.6258 |
| LEAP-Seq n confirming: | 7720 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTACCTCTGCCCGCATC |
| Suggested primer 2: | GCCCATGTTCTTACGTGCTT |