| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181633 |
| Chromosome: | chromosome 2 |
| Location: | 3232601 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095074 | VPS11,ROC12 | (1 of 1) PTHR23323//PTHR23323:SF24 - VACUOLAR MEMBRANE PROTEIN RELATED // VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG; Subunit of VPS-C complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCATTGAACAGTTCTAATCTGCCATGCG |
| Internal bar code: | GTGGGGACAATTTCACGCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 99.4379 |
| LEAP-Seq n confirming: | 3892 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAATGAACGTAACGCCCG |
| Suggested primer 2: | CGCCTAAGGCTTACTGATGC |