| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.181761 |
| Chromosome: | chromosome 12 |
| Location: | 4916918 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525500 | GCP2 | Gamma tubulin interacting protein; (1 of 1) K16569 - gamma-tubulin complex component 2 (TUBGCP2, GCP2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCTTGTGTCTCCATTGAACCCTCCATT |
| Internal bar code: | CTGCATAGCGTCAGACGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 99.7108 |
| LEAP-Seq n confirming: | 2758 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCATACCGGTACTCCTTA |
| Suggested primer 2: | CTTGGACAGCAGCTTAAGGG |