Insertion junction: LMJ.RY0402.181829_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.181829
Chromosome:chromosome 3
Location:1431437
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g151250 LANC1,LAN1 (1 of 1) PF05147 - Lanthionine synthetase C-like protein (LANC_like); LanC lantibiotic synthetase component C-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAATCACATACCAGTAGCCCTCCTTTACT
Internal bar code:AAATGCCACGCTAATCCTTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:814
LEAP-Seq percent confirming:99.7215
LEAP-Seq n confirming:1790
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGACTGCTCGAATGTTGAGG
Suggested primer 2:TCTTGCTAGGTGCGACATTG