Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.181847 |
Chromosome: | chromosome_12 |
Location: | 9068026 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g542950 | CGL137 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAAAGCACATTCAAGTAGCGCCCTTGCGCG |
Internal bar code: | GAGCGACGTCGGGAGGAACGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 428 |
LEAP-Seq percent confirming: | 94.0385 |
LEAP-Seq n confirming: | 4543 |
LEAP-Seq n nonconfirming: | 288 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGGGTTGAGGTATGCTAGG |
Suggested primer 2: | CACATCCTTTCGCAGTAGCA |