| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.181870 |
| Chromosome: | chromosome 16 |
| Location: | 7277153 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683371 | ODC2 | (1 of 2) 4.1.1.17 - Ornithine decarboxylase / L-ornithine carboxy-lyase; Ornithine decarboxylase 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGGCGTTCACTAGTAGGCAATGCATGG |
| Internal bar code: | TTCGTATTCGGTGATGCAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1042 |
| LEAP-Seq percent confirming: | 99.5135 |
| LEAP-Seq n confirming: | 12068 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTGTTTGGTAGTCCTGT |
| Suggested primer 2: | ATGTCTTGCCCCTTACATGC |