| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182077 |
| Chromosome: | chromosome 7 |
| Location: | 2479100 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329300 | MSC1 | Mechanosensitive ion channel; (1 of 1) PTHR30221 - SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACTGAAAACTGATGAGAGTGTTTGACA |
| Internal bar code: | AGGTCGCTCAGGCTGAGGAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 83.5137 |
| LEAP-Seq n confirming: | 2006 |
| LEAP-Seq n nonconfirming: | 396 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCAATGGGGATACGCAG |
| Suggested primer 2: | AACTCGGTTTGCAAAAGTCG |