Insertion junction: LMJ.RY0402.183371_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.183371
Chromosome:chromosome 8
Location:601320
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g359650 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAGTCGAGAAATGCAAACGCGACGGCGC
Internal bar code:GGGGGGCGGGCTAAGGCCGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:47
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:128
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCGGGGTTACTTTATGAG
Suggested primer 2:TCATCGAGGAGTGAGTGACG