| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183424 |
| Chromosome: | chromosome_9 |
| Location: | 3827621 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g391801 | VSP4 | Hydroxyproline-rich glycoprotein, cell wall protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTCAACCCAAGGCGCTCGCACTCCGGAGG |
| Internal bar code: | GCGCACGGGCGCGCGCGCTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 99.7089 |
| LEAP-Seq n confirming: | 1370 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAGGAGGAGTGAGGGCT |
| Suggested primer 2: | CCAGCAAGGAGGATGACAAT |