| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184049 |
| Chromosome: | chromosome 3 |
| Location: | 2295989 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158200 | HDA2 | (1 of 1) K11418 - histone deacetylase 11 (HDAC11); RPD3/HDA1 type histone deacetylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAATCGTACGTCCCCAGCCCACCCATCA |
| Internal bar code: | ACTTGGTATAATACCTTCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 99.4475 |
| LEAP-Seq n confirming: | 180 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTATGGATGTGTGTGCG |
| Suggested primer 2: | GCAAGAGCAAGGACAAGGAC |