Insertion junction: LMJ.RY0402.184071_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.184071
Chromosome:chromosome 4
Location:1921191
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g218150 PPH1,PPP18 Thylakoid phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCAAACCCCTCCCATCCCCAAACCCCCC
Internal bar code:GGTATTGGTGTTCCCAAGCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:32
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTCGGAACCACAAGAATG
Suggested primer 2:ATGTGCGTGTTTGTGTGGTT