| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184107 |
| Chromosome: | chromosome 12 |
| Location: | 9167698 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542250 | TUB1,RSP19 | (1 of 2) K07375 - tubulin beta (TUBB); beta-1 tubulin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTCGCTCCCGCGTCCCAGTCCCCCCGC |
| Internal bar code: | GGAGGGAATACGGGAGACAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 99.8638 |
| LEAP-Seq n confirming: | 1466 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTCTCGCACCCAAACAT |
| Suggested primer 2: | GCGGTCAACTCAAGGAATGT |