| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184135 |
| Chromosome: | chromosome 1 |
| Location: | 6646156 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047600 | SDR4 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.121 - Aldose 1-dehydrogenase (NAD(+)) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAACGCCGATGAAGTCGTGCCAACTGGC |
| Internal bar code: | TTTTCGGAGCTGAGTCCCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 80.7692 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCAAGCAGGACTGGAAC |
| Suggested primer 2: | ATTCGCGTACAGTGCAAGTG |