| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184756 |
| Chromosome: | chromosome 3 |
| Location: | 4693319 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177700 | NIT2 | Transcription factor regulating nitrogen metabolism; (1 of 1) IPR001932//IPR003035 - PPM-type phosphatase domain // RWP-RK domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCGCCGTTGTTGTAAGCCATCCCTGT |
| Internal bar code: | GAGCAGGACAGCTCACGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1134 |
| LEAP-Seq percent confirming: | 99.784 |
| LEAP-Seq n confirming: | 3234 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGGGTGGTGTCAAAAGC |
| Suggested primer 2: | CAGTAGAGGGGTCGAAGCTG |