| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184852 |
| Chromosome: | chromosome 12 |
| Location: | 2025649 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510050 | CTH1,CHL27B,CTH1B,CTH1A | Copper target homolog 1, chloroplast precursor%252C functional variant; (1 of 2) 1.14.13.81 - Magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase / Mg-protoporphyrin IX monomethyl ester oxidative cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGACCAAGAACCGCAAGTACACCTTCT |
| Internal bar code: | GTTTGTTTGATCTTTGGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 99.5042 |
| LEAP-Seq n confirming: | 2810 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGGGCGTTGTACTTGAC |
| Suggested primer 2: | AGGTTGAGGGCTTCAAGGTT |