Insertion junction: LMJ.RY0402.184873_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.184873
Chromosome:chromosome_1
Location:7071191
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre01.g051050 FAP225 Flagellar Associated Protein sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GTTGCAGGATGGCGCCTGTCCGCAGTACTA
Internal bar code:TAATTTTAACGGTTCGTCGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:664
LEAP-Seq percent confirming:87.5519
LEAP-Seq n confirming:211
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCGACCCACATACATAG
Suggested primer 2:CACAGATTCAGAGGCACGAA