| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184873 |
| Chromosome: | chromosome 1 |
| Location: | 7071191 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051050 | FAP225 | (1 of 18) PTHR10891 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN; Flagellar Associated Protein 225 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCAGGATGGCGCCTGTCCGCAGTACTA |
| Internal bar code: | TAATTTTAACGGTTCGTCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 87.5519 |
| LEAP-Seq n confirming: | 211 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCGACCCACATACATAG |
| Suggested primer 2: | CACAGATTCAGAGGCACGAA |