Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.185045 |
Chromosome: | chromosome_7 |
Location: | 1245404 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g321400 | FAP113 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCATGACCAGATGTTGAGCTACTTGATGC |
Internal bar code: | TTGAGGTCAGAACAGGCGTCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 570 |
LEAP-Seq percent confirming: | 96.645 |
LEAP-Seq n confirming: | 13798 |
LEAP-Seq n nonconfirming: | 479 |
LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCGTAATCGTGCTCGTCAG |
Suggested primer 2: | AAAGCCCCACGCCTAGTAAT |