Insertion junction: LMJ.RY0402.185045_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185045
Chromosome:chromosome 7
Location:1245404
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCATGACCAGATGTTGAGCTACTTGATGC
Internal bar code:TTGAGGTCAGAACAGGCGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:96.645
LEAP-Seq n confirming:13798
LEAP-Seq n nonconfirming:479
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGTAATCGTGCTCGTCAG
Suggested primer 2:AAAGCCCCACGCCTAGTAAT