| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185045 |
| Chromosome: | chromosome_7 |
| Location: | 1245416 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g321400 | FAP113 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGAGAGAGGATGTTGAGTAAGGTTCGGC |
| Internal bar code: | GGAGTGGCTTGCGATCGGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 574 |
| LEAP-Seq percent confirming: | 96.345 |
| LEAP-Seq n confirming: | 659 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTAATCGTGCTCGTCAG |
| Suggested primer 2: | AAAGCCCCACGCCTAGTAAT |