Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.187060 |
Chromosome: | chromosome 10 |
Location: | 1325109 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g427650 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCAGGCGCCCCTCCAGGCTGACAGCC |
Internal bar code: | GTAGGCGCGAACTATCATTGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 401 |
LEAP-Seq percent confirming: | 99.6169 |
LEAP-Seq n confirming: | 520 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGTCATAGCCAAAGCAGCC |
Suggested primer 2: | AGCAGGTTGCTCTGTGAGGT |