| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.187336 |
| Chromosome: | chromosome 12 |
| Location: | 8377990 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549100 | UAF3,SPL10 | splicing factor U2AF 65 kDa subunit; (1 of 2) PTHR23139//PTHR23139:SF58 - RNA-BINDING PROTEIN // SUBFAMILY NOT NAMED | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGGGGTAACGCCTCGGCAATCGTGTCG |
| Internal bar code: | CGGGGCGCGTGTTATCGGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 135 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTCAGCGCACTAGGAAA |
| Suggested primer 2: | ATCTTTCCTCACACCATCGC |