| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189225 |
| Chromosome: | chromosome 9 |
| Location: | 4282813 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394621 | (1 of 8) IPR002044//IPR013784 - Carbohydrate binding module family 20 // Carbohydrate-binding-like fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCGGTGCCGCGTGCCTTGGCAGTTGTG |
| Internal bar code: | TTCGGCTTTATCTTATAGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 99.6124 |
| LEAP-Seq n confirming: | 2313 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACGCTACGCGAGATCAT |
| Suggested primer 2: | AAAACTCTGGGCCGTAACCT |