Insertion junction: LMJ.RY0402.189982_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.189982
Chromosome:chromosome 11
Location:981911
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467665 (1 of 1) IPR002909//IPR006626//IPR011050//IPR014756//IPR019316 - IPT domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Immunoglobulin E-set // G8 domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTACACTAGGTGGATGTGATGGCTCAG
Internal bar code:GACGGCAAGGGAATATTGATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:780
LEAP-Seq percent confirming:98.9313
LEAP-Seq n confirming:648
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AATCATCATCCCACTCGCA
Suggested primer 2:ACTCCTGCGCAGCATCTATC