| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190806 |
| Chromosome: | chromosome 6 |
| Location: | 4894503 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279150 | TSD2 | (1 of 2) 6.1.1.12 - Aspartate--tRNA ligase / Aspartyl-tRNA synthetase; Aspartyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTAGAGGTGTGCAGGTACGGCGAGGCA |
| Internal bar code: | GTACCGTGGAGGGCTTAAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1058 |
| LEAP-Seq percent confirming: | 98.9351 |
| LEAP-Seq n confirming: | 2044 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGGACTGGCAACACCTT |
| Suggested primer 2: | GACTTCAACCGCTTCTTTGC |