| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191327 |
| Chromosome: | chromosome 12 |
| Location: | 1285139 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489150 | SHD1 | SH2-domain protein; (1 of 2) IPR000980 - SH2 domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTTGACCGTCCACTCCTTGCAACCGTA |
| Internal bar code: | TTAGGCCTCTCCTCGGGGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAACCGTGTGTTTGGGCT |
| Suggested primer 2: | CACACAGACACACTGCAACG |