Insertion junction: LMJ.RY0402.191399_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.191399
Chromosome:chromosome 6
Location:8466192
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g307500 LCIC,LCIC1 Low-CO2 inducible protein C 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTTGAGCTCGAGATGCTCTCATCCGTC
Internal bar code:GAAACCGGCCCGGTCAGCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:300
LEAP-Seq percent confirming:99.6993
LEAP-Seq n confirming:1658
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAGGGATGGGAACTTGAG
Suggested primer 2:CGGTGATGATGGTGTGGTAG