| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191736 |
| Chromosome: | chromosome 5 |
| Location: | 1944925 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241635 | (1 of 1) IPR012344//IPR014001//IPR027417 - Matrix protein, lentiviral and alpha-retroviral // Helicase superfamily 1/2, ATP-binding domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTGCGAGACACGCATACCACGTGTACC |
| Internal bar code: | GCGTAGTCAAAAGAACGGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 25 |
| LEAP-Seq percent confirming: | 11.2972 |
| LEAP-Seq n confirming: | 290 |
| LEAP-Seq n nonconfirming: | 2277 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGGCACGATGCAAGTAA |
| Suggested primer 2: | GTTGCGTAGTGATGGTGGTG |