Insertion junction: LMJ.RY0402.192273_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.192273
Chromosome:chromosome 16
Location:6157078
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g675500 FKB16-5,FKB8,FKB16E Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 25) PTHR10516 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGACGTTCAGGTTGAAGTGACCAGAGTC
Internal bar code:GACAGCCGCTCGAGAAGGGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:970
LEAP-Seq percent confirming:97.0267
LEAP-Seq n confirming:2219
LEAP-Seq n nonconfirming:68
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACATCATCATCAGCCAG
Suggested primer 2:GTATGGAGACAGCGTGAGCA