| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192784 |
| Chromosome: | chromosome 9 |
| Location: | 2887532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388372 | RAT2,OPR39 | tscA RNA stabilization factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATGTAAACACAGAAATGTTAGTAAGTT |
| Internal bar code: | GGGTAGGGGGTTATATTACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 431 |
| LEAP-Seq percent confirming: | 99.2023 |
| LEAP-Seq n confirming: | 1368 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTGAAAGAGCTGGGTGC |
| Suggested primer 2: | CGCTTACTCCACCCTACAGC |