| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192888 |
| Chromosome: | chromosome 13 |
| Location: | 1483687 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572500 | COA2,CGL14 | (1 of 1) PTHR12280//PTHR12280:SF31 - PANTOTHENATE KINASE // SUBFAMILY NOT NAMED; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCAGACCCCGGGCACACACACCTCTCG |
| Internal bar code: | CCGTGGATGGTCTGGGAACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 86.419 |
| LEAP-Seq n confirming: | 2768 |
| LEAP-Seq n nonconfirming: | 435 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAAACCGACCCAAGCTCA |
| Suggested primer 2: | ATTGCGGACATTGCACATAA |