| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193315 |
| Chromosome: | chromosome_1 |
| Location: | 3907803 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025400 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACCGTGCAGCGCTACCGGACCGAGCTGGT |
| Internal bar code: | GAGCTGGGGCTCTTGGATGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 88.0476 |
| LEAP-Seq n confirming: | 1849 |
| LEAP-Seq n nonconfirming: | 251 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGTACGAGGTGGAGCTG |
| Suggested primer 2: | CGAAGAAGGAGCTGGACAAC |