| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.193348 |
| Chromosome: | chromosome 12 |
| Location: | 8150363 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551200 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCCTCTTCACTGCCGCATAATACGCTC |
| Internal bar code: | GTGGGGTCAATACGTCTCTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 0.0 |
| LEAP-Seq n confirming: | 0 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 0 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTTATCCCACGTGCAAA |
| Suggested primer 2: | CCGAATACGTGTTCTCCGTT |