| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.193348 |
| Chromosome: | chromosome 12 |
| Location: | 8150367 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551200 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGCGCTTCTGGCTATGCTGCTTACCTT |
| Internal bar code: | ATCATCGACCCTTTTTGTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 582 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAATACGTGTTCTCCGTT |
| Suggested primer 2: | TGTTTTATCCCACGTGCAAA |