| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193500 |
| Chromosome: | chromosome 10 |
| Location: | 2676033 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437829 | CIA6,CGL89 | (1 of 1) PTHR13271//PTHR13271:SF19 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // PROTEIN SET DOMAIN GROUP 40; methyltransferase domain protein involved in pyrenoid biogenesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTCAGGTGAGGTGGAGCCATCATTCG |
| Internal bar code: | GTGAATCGTCCCGTTCGGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.5862 |
| LEAP-Seq n confirming: | 722 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCAGGTCCTCAAGCTG |
| Suggested primer 2: | GGATCAGTTTATGGAGCGGA |