| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.193500 |
| Chromosome: | chromosome 10 |
| Location: | 2676037 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437829 | CIA6,CGL89 | (1 of 1) PTHR13271//PTHR13271:SF19 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // PROTEIN SET DOMAIN GROUP 40; methyltransferase domain protein involved in pyrenoid biogenesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATCCAGGCGCACTGTCCATGGAACTGCA |
| Internal bar code: | CGGCCGGCCCCTTTCGCACTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 97.2136 |
| LEAP-Seq n confirming: | 628 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCAGGTCCTCAAGCTG |
| Suggested primer 2: | GGATCAGTTTATGGAGCGGA |