Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.193945 |
Chromosome: | chromosome_13 |
Location: | 4173913 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g592150 | SPL28,PRP28 | DEAD/DEAH box helicase, possible nuclear pre-mRNA splicing facto | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCCTGAACCCGCCGCCACTTCCCGCCAGC |
Internal bar code: | CGGGAAAGGCAGGAGTAGCACGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 213 |
LEAP-Seq percent confirming: | 94.8718 |
LEAP-Seq n confirming: | 481 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTTGGAGTCCCAGTCAAAA |
Suggested primer 2: | AGAAGCAGCAGCAGGAAGAG |