| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193970 |
| Chromosome: | chromosome 1 |
| Location: | 6037507 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042800 | DVR1 | 3%252C8-Divinyl protochlorophyllide a 8-vinyl reductase, chloroplast precursor; (1 of 1) K19073 - divinyl chlorophyllide a 8-vinyl-reductase (DVR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTAATGTTGCATGGAGGGCGCGCACTA |
| Internal bar code: | ACTAGGTGTGGGGCGTGGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 74.4636 |
| LEAP-Seq n confirming: | 1423 |
| LEAP-Seq n nonconfirming: | 488 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTATCCCTGACACCTGTT |
| Suggested primer 2: | GGGTTGGTGGCTGAAGATAA |