| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.194206 |
| Chromosome: | chromosome 12 |
| Location: | 7636416 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554800 | PRK1 | (1 of 1) 2.7.1.19 - Phosphoribulokinase / Phosphopentokinase; Phosphoribulokinase, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGTCGTCCACCACAGCTACAGCTAACA |
| Internal bar code: | CCCTACGGTTAGTTGCTGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 167 |
| LEAP-Seq percent confirming: | 99.1667 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGCTCAGGTGAAGGAG |
| Suggested primer 2: | AAGGCAAAGCAAGTGCCTAA |