| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194306 |
| Chromosome: | chromosome 16 |
| Location: | 1928646 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656150 | (1 of 1) PTHR23504:SF1 - PROTEIN T25D3.4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTATTATATAGTTTCTGTGTGCTACTC |
| Internal bar code: | ACAACGTTCGCCGGCCGAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 98.8219 |
| LEAP-Seq n confirming: | 2097 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAAGTTTGTCCAGCTCA |
| Suggested primer 2: | ACGCACCTTAGCAGCAAAAT |