| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195279 |
| Chromosome: | chromosome 13 |
| Location: | 3271734 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586050 | (1 of 1) PTHR13833:SF49 - NHL REPEAT-CONTAINING PROTEIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGCGCTGTGCACGCCCACCACACACAC |
| Internal bar code: | GTTTGAACGCTTCGTTCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 95.8851 |
| LEAP-Seq n confirming: | 6944 |
| LEAP-Seq n nonconfirming: | 298 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTTGGTTTAGCTTGGG |
| Suggested primer 2: | GCGTTGACTGCTGTGTTTGT |