Insertion junction: LMJ.RY0402.195781_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.195781
Chromosome:chromosome 2
Location:3243770
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095077 (1 of 1) PF00571//PF04134 - CBS domain (CBS) // Protein of unknown function, DUF393 (DUF393) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTATGGCGCCTTTGGCAAGGGAGGTTGA
Internal bar code:GTGGTAAACGCTCGCTTACCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGACACCGAAGAGGATGG
Suggested primer 2:GCAGCAGCAGCAGTAGTACG