| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196268 |
| Chromosome: | chromosome 3 |
| Location: | 5389617 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184651 | RAD51D | DNA recombination protein; (1 of 1) PTHR22942:SF29 - DNA REPAIR PROTEIN RAD51 HOMOLOG 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTCGCCTCAACTTAGCCGTGCCCGCTC |
| Internal bar code: | TCACTGTGACAGGCGCGCCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.9321 |
| LEAP-Seq n confirming: | 4413 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACGATAGTATGCACCG |
| Suggested primer 2: | GCTTTAAATGCAGAGACGCC |