| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196279 |
| Chromosome: | chromosome 10 |
| Location: | 1023379 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425050 | ROC59,ROC28 | (1 of 1) K11807 - WD and tetratricopeptide repeats protein 1 (WDTC1); Rhythm Of Chloroplast 59 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACAGCTGAGTCGCTGATGATTCGGCTT |
| Internal bar code: | GGTTTTCCGACAAGATGCTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 617 |
| LEAP-Seq percent confirming: | 99.5787 |
| LEAP-Seq n confirming: | 2836 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTCTGTGCTCACCCAAA |
| Suggested primer 2: | GCTGTGGCCGTTATAGGTGT |