| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196583 |
| Chromosome: | chromosome 6 |
| Location: | 1568543 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260450 | LCI20 | Putative 2-oxoglutarate/malate translocator; (1 of 1) K03319 - divalent anion:Na+ symporter, DASS family (TC.DASS) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGCATATTGCAAGGGTTTAGGAAGTCA |
| Internal bar code: | GGCCGGAGTTAGAGGCTGCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 870 |
| LEAP-Seq percent confirming: | 99.7006 |
| LEAP-Seq n confirming: | 2664 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGTTTCCGCAGTAGAAC |
| Suggested primer 2: | ACATCATCTCTATTGCCCCG |