Insertion junction: LMJ.RY0402.197408_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.197408
Chromosome:chromosome 3
Location:2296678
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g158200 HDA2 (1 of 1) K11418 - histone deacetylase 11 (HDAC11); RPD3/HDA1 type histone deacetylase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGATGACGACGAATCCCCCCCCCCCCAT
Internal bar code:TAAAACCGTTGACAGTACACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:249
LEAP-Seq percent confirming:90.708
LEAP-Seq n confirming:205
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGTCTGTGGTACAATGG
Suggested primer 2:CTGCAACTAAATCGGTGGGT