Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.197498 |
Chromosome: | chromosome_3 |
Location: | 4559386 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g176833 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAAAAGTGCGGCGAACGCCACGCCCTAACG |
Internal bar code: | AGGTGGTTTCCTGGCGCCCGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 437 |
LEAP-Seq percent confirming: | 88.5851 |
LEAP-Seq n confirming: | 1296 |
LEAP-Seq n nonconfirming: | 167 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGGGTAGTCCAAAGCTCA |
Suggested primer 2: | ACACCGCATCATGTTCGTAA |