| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.197728 |
| Chromosome: | chromosome 17 |
| Location: | 2818792 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719000 | NSG12,UAP56 | Exon junction complex component, UAP56 DEAD/DEAH box helicase ho | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTTGTGTTAGCTGTCAGGACTACTCACT |
| Internal bar code: | AAGGATTCGACAGCGGCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1037 |
| LEAP-Seq percent confirming: | 99.7515 |
| LEAP-Seq n confirming: | 2007 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCCGATAGTCACACCCT |
| Suggested primer 2: | TCGTGTTATAACTTCCCCGC |