| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198063 |
| Chromosome: | chromosome 12 |
| Location: | 5116457 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526950 | (1 of 1) K03130 - transcription initiation factor TFIID subunit 5 (TAF5); Transcription initiation factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCCCCCCCCCCCCCCCCCCCCCCCCCC |
| Internal bar code: | TCGGGCGGGATATTGCCCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTCATGCCTACGTGTTG |
| Suggested primer 2: | ATGTCCACCAGGGTCAGGTA |