| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198556 |
| Chromosome: | chromosome 10 |
| Location: | 1013339 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425050 | ROC59,ROC28 | (1 of 1) K11807 - WD and tetratricopeptide repeats protein 1 (WDTC1); Rhythm Of Chloroplast 59 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGTGTAAAAGGGCTAGCGCTGGAAAAA |
| Internal bar code: | TTGGCCTCCAGACGCTTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1111 |
| LEAP-Seq percent confirming: | 97.5746 |
| LEAP-Seq n confirming: | 2977 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGTTGTCAGTGAGGGTT |
| Suggested primer 2: | GTTGACGTTGACGTTGATGG |