| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199179 |
| Chromosome: | chromosome 9 |
| Location: | 679225 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402950 | CGL21 | Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCTACACCCTACATCCTGGAAACCGC |
| Internal bar code: | CAAATCGTCCAACGTATTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 99.709 |
| LEAP-Seq n confirming: | 1028 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCACTGTTATTGGGACC |
| Suggested primer 2: | CGCCACCATACCGTAGTTCT |